The C-terminal region (640–967) of Arabidopsis CPL1 interacts with the abiotic stress- and ABA-responsive transcription factors
- 作者：Woo Young Bang ; Se Won Kim ; In Sil Jeong ; Hisashi Koiwa ; Jeong Dong Bahk
- 关键词：AtCPL1 ; AtMYB3 ; ANAC019 ; CTD phosphatase ; dsRNA-binding motif
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2008
- 期刊代码：159_0006291x
- 类别：bio
- 出版时间：8 August 2008
- 卷：372
- 期：4
- 页码：907-912
- 文件大小：1271 K
摘要
Proteins in CPL1 family are unique to plants and contain a phosphatase catalytic domain and double-stranded RNA (dsRNA)-binding motifs (DRMs) in a single peptide. Though DRMs are important for the function of Arabidopsis CPL1 in vivo, the role of CPL1 DRM has been obscure. We have isolated two transcription factors, ANAC019 (At1g52890) and AtMYB3 (At1g22640), which specifically interact with the C-terminal region (640–967) of AtCPL1 containing two DRMs. Detailed interaction analysis indicated that AtMYB3 specifically interacted with the first DRM but not with the second DRM in CPL1 C-terminal fragment. GFP-fusion analysis indicated that AtMYB3 localized in nuclei-like CPL1, and its expression is induced by abiotic stress and ABA treatment. These results suggest that AtMYB3 function in abiotic stress signaling in concert with CPL1.