Transformation-associated gene regulation by ATF6α during hepatocarcinogenesis
- 作者：Masaaki Arai ; Nobuo Kondoh ; Nobuo Imazeki ; Akiyuki Hada ; Kazuo Hatsuse ; Fumihiro Kimura ; Osamu Matsubara ; Kazutoshi Mori ; Toru Wakatsuki and Mikio Yamamoto
- 关键词：ER stress ; Unfolded protein response ; Liver cancer ; ATF6
- 刊名：FEBS Letters
- 出版年：2006
- 期刊代码：70_00145793
- 类别：bio
- 出版时间：9 January 2006
- 卷：580
- 期：1
- 页码：184-190
- 文件大小：230 K
摘要
We have previously reported that the endoplasmic reticulum (ER) stress-regulated transmembrane transcription factor 6 α (ATF6α) is implicated in the pathogenesis of hepatocellular carcinomas (HCCs). In order to further identify genes that are regulated by ATF6α, the global gene expression profiles of the ATF6α-transfected and untransfected HCC cell line, HLF, were analyzed. These results were then compared with the differential gene expression patterns of poorly differentiated HCC and control non-tumorous liver tissue. Our findings demonstrate that at least 18 genes are specifically upregulated by ATF6α, while another UPR mediator, XBP1 or ER-stress inducer, thapsigargin could partially stimulate or even repress some of them in HCC cells. Moreover, six of these identified genes contain potential ER stress-responsive elements and/or unfolded protein response elements in their 5′ regulatory regions.