Peroxiredoxin-4 interacts with and regulates the thromboxane A₂ receptor

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• 作者：Patrick Giguè ; re ; Marie-Eve Turcotte ; Emilie Hamelin ; Audrey Parent ; Jessy Brisson ; Geneviè ; ve Laroche ; Pascale Labrecque ; Gilles Dupuis ; Jean-Luc Parent
• 关键词：GPCR ; G protein-coupled receptor ; Peroxiredoxin ; Oxidative stress ; Trafficking ; Signalling
• 刊名：FEBS Letters
• 出版年：2007
• 期刊代码：70_00145793
• 类别：bio
• 出版时间：7 August 2007
• 卷：581
• 期：20
• 页码：3863-3868
• 文件大小：469 K

摘要

We identified peroxiredoxin-4 (Prx-4) as a protein interacting with the β isoform of the thromboxane A₂ receptor (TPβ) by yeast two-hybrid analysis. Prx-4 co-immunoprecipitated constitutively with TPβ in HEK293 cells. The second and third intracellular loops as well as the C-terminus of TPβ interacted directly with Prx-4. Co-expression of Prx-4 caused a 60%decrease in cell surface expression of TPβ. Prx-4 and TPβ predominantly co-localized in the endoplasmic reticulum. Co-expression of Prx-4 in cells treated with H₂O₂ targeted TPβ for degradation. We show for the first time an interaction between a receptor involved in oxidative stress and Prx-4, an anti-oxidative enzyme.