Longevity of crapemyrtle pollen stored at different temperatures

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• 作者：A.S.M.G. Masum Akond^a ; Cecil T. Pounders^b ; ^{Cecil.Pounders@ARS.USDA.GOV} ; Eugene K. Blythe^c ; Xinwang Wang^a ; ^{xw-wang@tamu.edu}
• 关键词：Lagerstroemia spp. ; Pollen germination ; Pollen transportation ; Interspecific cross ; Breeding
• 刊名：Scientia Horticulturae
• 出版年：2012
• 期刊代码：93_03044238
• 类别：abs
• 出版时间：18 May, 2012
• 卷：139
• 期：Complete
• 页码：53-57
• 文件大小：285 K

摘要

Temperatures for storage of crapemyrtle (Lagerstroemia spp.) pollen over time were studied using clones of two interspecific hybrids (L. 鈥楥heyenne鈥?and L. 鈥榃ichita鈥? and five species (L. indica 鈥楥atawba鈥? L. subcostata (NA 40181), L. limii (SHL2004-1), L. speciosa (MIA 36606), and L. fauriei 鈥楰iowa鈥?. Pollen samples were stored at room temperature (23 卤 1 掳C), 4 掳C, 鈭?0 掳C and 鈭?0 掳C. Germination of pollen samples was examined at 0, 7, 15, 45, 75, or 105 days of storage (DOS) and data were analyzed using linear models. Fresh (0 DOS) pollen of L. 鈥楥heyenne鈥?showed the highest germination (80%). For all other clones, pollen germination at 0 DOS ranged from 44%(L. speciosa) to 77%(L. fauriei 鈥楰iowa鈥?. Pollen of all clones lost their viability within 7 DOS at room temperature. Overall, this study indicated that Lagerstroemia pollen is best maintained over time when pollen is stored under refrigeration, with a storage temperature of 鈭?0 掳C often being preferable to 4 掳C. Lowering the storage temperature to 鈭?0 掳C is generally unnecessary, but not deleterious to pollen germination. Storage of viable crapemyrtle pollen for no longer than 75-105 days is adequate for allowing breeders to efficiently execute hybridization of germplasm flowering at different times or in widely separated locations.