Mitochondrial calcium response in human transformed lymphoblastoid cells

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• 作者：Kato ; Tadafumi ; Ishiwata ; Mizuho ; Nagai ; Takeharu
• 关键词：Calcium ; Mitochondria ; Platelet activating factor ; Thapsigargin ; Carbonyl cyanide m-chlorophenylhydrazone
• 刊名：Life Sciences
• 出版年：2002
• 期刊代码：62_00243205
• 类别：imm
• 出版时间：June 21, 2002
• 卷：71
• 期：5
• 页码：581-590
• 文件大小：224 K

摘要

Human lymphoblastoid cell line (LCL) transformed by Epstein-Barr Virus (EBV) is a unique cellular model for the study of human diseases. Although pathophysiological significance of mitochondrial calcium regulation is drawing attention, it is not known whether or not mitochondria in LCLs play a role in intracellular calcium signaling. In this study, role of mitochondria of the lymphoblastoid cell line in calcium signaling was examined. Intra-mitochondrial calcium concentration ([Ca²⁺]m) was successfully measured using dihydro-Rhod-2, revealed by the decrease of fluorescence after application of carbonyl cyanide m-chlorophenylhydrazone (CCCP) and intracellular localization patterns imaged by florescent microscope. Platelet activating factor (PAF) concentration-dependently increased cytosolic calcium concentration ([Ca²⁺]i), while no increase of [Ca²⁺]m was observed. In contrast, 10 μM thapsigargin increased [Ca²⁺]i as well as [Ca²⁺]m. LCLs may be used for the study of possible pathophysiological role of mitochondrial calcium regulation in human diseases.