Adipogenesis-related increase of semicarbazide-sensitive amine oxidase and monoamine oxidase in human adipocytes
- 作者：S ; y Bour ; Daniè ; le Daviaud ; S ; ra Gres ; Corinne Lefort ; Danielle Pré ; vot ; Antonio Zorzano ; Martin Wabitsch ; Jean-Sé ; bastien Saulnier-Blache ; Philippe Valet ; Christian Carpé ; né
- 关键词：Insulin ; Amine oxidase ; Adipocyte differentiation ; Adipogenesis ; Obesity
- 刊名：Biochimie
- 出版年：2007
- 期刊代码：24_03009084
- 类别：bio
- 出版时间：August 2007
- 卷：89
- 期：8
- 页码：916-925
- 文件大小：543 K
摘要
A strong induction of semicarbazide-sensitive amine oxidase (SSAO) has previously been reported during murine preadipocyte lineage differentiation but it remains unknown whether this emergence also occurs during adipogenesis in man. Our aim was to compare SSAO and monoamine oxidase (MAO) expression during <i>in vitroi> differentiation of human preadipocytes and in adipose and stroma-vascular fractions of human fat depots. A human preadipocyte cell strain from a patient with Simpson–Golabi–Behmel syndrome was first used to follow amine oxidase expression during <i>in vitroi> differentiation. Then, human preadipocytes isolated from subcutaneous adipose tissues were cultured under conditions promoting <i>ex vivoi> adipose differentiation and tested for MAO and SSAO expression. Lastly, human adipose tissue was separated into mature adipocyte and stroma-vascular fractions for analyses of MAO and SSAO at mRNA, protein and activity levels. Both SSAO and MAO were increased from undifferentiated preadipocytes to lipid-laden cells in all the models: 3T3-F442A and 3T3-L1 murine lineages, human SGBS cell strain or human preadipocytes in primary culture. In human subcutaneous adipose tissue, the adipocyte-enriched fraction exhibited seven-fold higher amine oxidase activity and contained three- to seven-fold higher levels of mRNAs encoded by <i>MAO-A, MAO-B, AOC3i> and <i>AOC2i> genes than the stroma-vascular fraction. <i>MAO-Ai> and <i>AOC3i> genes accounted for the majority of their respective MAO and SSAO activities in human adipose tissue. Most of the SSAO and MAO found in adipose tissue originated from mature adipocytes. Although the mechanism and role of adipogenesis-related increase in amine oxidase expression remain to be established, the resulting elevated levels of amine oxidase activities found in human adipocytes may be of potential interest for therapeutic intervention in obesity.