Does Sumoylation Control K2P1/TWIK1 Background K⁺ Channels?

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• 作者：Sylvain Feliciangeli ; Saï ; d Bendahhou ; Guillaume S ; oz ; Pierre Gounon ; Markus Reichold ; Richard Warth ; Michel Lazdunski ; Jacques Barhanin ; Florian Lesage
• 关键词：PROTEINS ; SINGALING
• 刊名：Cell
• 出版年：2007
• 期刊代码：50_00928674
• 类别：med
• 出版时间：10 August 2007
• 卷：130
• 期：3
• 页码：563-569
• 文件大小：498 K

摘要

A novel model for the regulation of cell excitability has recently been proposed. It originates from the observation that the background K⁺ channel K2P1 (TWIK1) may be silenced by sumoylation in Xenopus oocytes and that inactivation of the putative sumoylation site (mutation K274E) gives rise to robust current expression in transfected COS-7 cells. Here, we show that only the mutation K274E, and not K274R, is associated with an increase of K2P1 current density, suggesting a charge effect of K274E. Furthermore, we failed to observe any band shift by western blot analysis that would confirm an eventual sumoylation of K2P1 in COS-7 cells and oocytes.