Characterization of porcine T lymphocytes and their immune response against viral antigens
- 作者：Saalmü ; ller ; Armin ; Pauly ; Thomas ; Hö ; hlich ; Bettina-Judith ; Pfaff ; Eberhard
- 关键词：Differentiation antigens ; Porcine T lymphocytes ; T-helper cells ; Natural killer cells ; Cytolytic T lymphocytes ; Classical swine fever virus ; Pseudorabies virus
- 刊名：Journal of Biotechnology
- 出版年：1999
- 期刊代码：53_01681656
- 类别：imm
- 出版时间：August 20, 1999
- 卷：73
- 期：2-3
- 页码：223-233
- 文件大小：172 K
摘要
T lymphocytes play a central role in the antigen-specific immune response against various pathogens. To detect and to characterize porcine T lymphocytes, monoclonal antibodies (mAb) against leukocyte differentiation antigens had been raised and classified for their specificity. Analyses of porcine T lymphocytes with specific mAb against CD4 and CD8 differentiation antigens revealed differences in the composition of the porcine T-lymphocyte population compared to other species. In addition to the known subpopulations, CD4+CD8− T helper cells and CD4−CD8+ cytolytic T lymphocytes, extra-thymic CD4+CD8+ T lymphocytes and a substantial proportion of CD2−CD4−CD8− T cell receptor (TcR)-γδ+ T cells could be detected in swine. Functional analyses of porcine T-lymphocyte subpopulations revealed the existence of two T-helper cell fractions with the phenotype CD4+CD8− and CD4+CD8+. Both were reactive in primary immune responses in vitro, whereas only cells derived from the CD4+CD8+ T-helper-cell subpopulation were able to respond to recall antigen in a secondary immune response. With regard to T lymphocytes with cytolytic activities, two subsets within the CD4−CD8+ T-cell subpopulation could be defined by the expression of CD6 differentiation antigens: CD6− cells which showed spontaneous cytolytic activity and CD6+ MHC I-restricted cytolytic T lymphocytes including virus-specific cytolytic T lymphocytes. These results enable now a detailed view into the porcine T-cell population and the reactivity of specific T cells involved in the porcine immune response against pathogens. Furthermore this knowledge offers the possibility to investigate specific interactions of porcine T lymphocytes with virus-specific epitopes during vaccination and viral infections.