Proteolytic cleavage of annexin 1 by human leukocyte elastase
- 作者：Ursula Rescher ; Verena Goebeler ; Andreas Wilbers ; Volker Gerke
- 关键词：Calcium ; Endothelial cell ; Inflammation ; Elastase
- 刊名：Biochimica et Biophysica Acta (BBA)/Molecular Cell Research
- 出版年：2006
- 期刊代码：20_01674889
- 类别：bio
- 出版时间：November 2006
- 卷：1763
- 期：11
- 页码：1320-1324
- 文件大小：257 K
摘要
Annexin 1 has been shown to participate through its unique N-terminal domain in the recruitment and activation of leukocytes at sites of inflammation. Peptides derived from this domain are true mimetics of the annexin 1 action in all inflammation models tested and most likely serve as the active entities generated at sites of inflammation. To elucidate mechanisms underlying peptide generation we used isolated blood leukocytes and endothelial cell monolayers. We show that following endothelial adhesion, annexin 1 was externalized from leukocytes and rapidly cleaved. Addition of purified annexin 1 to degranulating leukocytes resulted in the truncation of annexin 1, which seemed to depend on the proteolytic activity of human leukocyte elastase (HLE). The capacity of elastase to proteolytically cleave annexin 1 was confirmed using both purified annexin 1 and HLE. The identification of annexin 1 as a substrate for HLE supports the model in which annexin 1 participates in regulating leukocyte emigration into inflamed tissue through N-terminal peptides generated at inflammatory sites.