Enhanced cell uptake of superparamagnetic iron oxide nanoparticles through direct chemisorption of FITC-Tat-PEG₆₀₀-b-poly(glycerol monoacrylate)

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• 作者：Chenhong Wang^a ; Lei Qiao^a ; Quan Zhang^a ; Husheng Yan^b ; ^{yanhs@nankai.edu.cn} ; Keliang Liu^a ; ^{keliangliu55@126.com} ; ^{keliangliu@yahoo.com}
• 关键词：Superparamagnetic iron oxide (SPIO) nanoparticle ; Multifunctional magnetic nanoparticle system ; FITC-Tat penetrating peptide
• 刊名：International Journal of Pharmaceutics
• 出版年：2012
• 期刊代码：24_03785173
• 类别：pha
• 出版时间：1 July, 2012
• 卷：430
• 期：1-2
• 页码：372-380
• 文件大小：1223 K

摘要

Magnetic nanoparticles (MNPs) functionalized with specific ligands are emerging as a highly integrated platform for cancer targeting, drug delivery, and magnetic resonance imaging applications. In this study, we describe a multifunctional magnetic nanoparticle system (FITC-Tat MNPs) consisting of a fluorescently labeled cell penetrating peptide (FITC-Tat peptide), a biocompatible block copolymer PEG₆₀₀-b-poly(glycerol monoacrylate) (PEG₆₀₀-b-PGA), and a superparamagnetic iron oxide (SPIO) nanoparticle core. The particles were prepared by direct chemisorption of PEG₆₀₀-b-PGA conjugated with FITC-Tat peptide on the SPIO nanoparticles. FITC-MNPs without Tat were prepared for comparison. Flow cytometry assays revealed significantly higher uptake of FITC-Tat MNPs compared to FITC-MNPs in Caco-2 cells. These results were confirmed using confocal laser scanning microscopy (LSCM), which further demonstrated that the FITC-Tat MNPs accumulated in the cytoplasm and nucleus while the FITC-MNPs were localized in the cell membrane compartments. The FITC-Tat MNPs did not exhibit observable cytotoxicity in MTS assays.