The telomerase activity and expression of hTERT gene can serve as indicators in the anti-cancer treatment of human ovarian cancer

详细信息	查看全文 | 推荐本文 |

• 作者：Peng-Ming Sun ; Li-Hui Wei ; Mei-Yu Luo ; Guang Liu ; Jiang-Liu Wang ; Alex ; er Mustea ; Dominique Kö ; nsgen ; Werner Lichtenegger ; Jaild Sehouli
• 关键词：Ovarian cancers ; Telomearase activity ; hTERT mRNA ; Biomarker ; Apoptosis
• 刊名：European Journal of Obstetrics and Gynecology and Reproductive Biology
• 出版年：2007
• 期刊代码：92_03012115
• 类别：med
• 出版时间：February 2007
• 卷：130
• 期：2
• 页码：249-257
• 文件大小：984 K

摘要

>Objective

The aim of this study was to evaluate the clinicopahtological significance of telomerase activity and expression of hTERT gene in human ovarian cancer. The potential value of them as indicators for chemotherapy in ovarian cancer cells was also studied.

Materials and methods

A total of 73 samples and ovarian cancer cell lines HO-8910 and COC₁ were studied. Telomerase activity was detected by PCR-TRAP-ELISA assay and the expression of the hTERT mRNA was analyzed by semi-quantitative RT-PCR. Alteration of the telomerase activity and hTERT mRNA were also analyzed in the ovarian cancer cells treated with different concentration and different time of cisplatin. Cytogenetic analysis was performed to compare the telomere status in the OH-8910 cells pre- and post-cisplatin treatment. The associations between these two markers and cisplatin induced-apoptosis were respectively analyzed in COC₁ cells by the flow cytometry.

Results

Telomerase activity are highly increased in malignancy (0.795 ± 0.168_{A450–655 nm}) than borderline (0.389 ± 0.174_{A450–655 nm}), benign tumors (0.236 ± 0.102_{A450–655 nm}) and normal ovary (0.213 ± 0.070_{A450–655 nm}) (p < 0.05). Twenty samples showed detectable levels of hTERT. The hTERT gene positive lesion showed significantly higher telomerase activity than negative (p = 0.004). There is a significant correlation between the telomerase activity and expression of hTERT (r = 0.921). Both telomerase activity and expression of hTERT can reflect the chemotherapeutic effect of cisplatin in a time-dependent and dose-dependent manner. Treatment with 10 μM cisplatin, the hTERT mRNA decreased after 12 h and completely disappeared after 48 h, whereas the telomerase activity did not decrease until 24 h. Results from cytogenetic analysis and flow cytometry assay confirmed that the alterations of these two markers are associated with the anti-cancer treatment of cisplatin.

Conclusion

Expression of hTERT gene is rate-limiting with the activation of telomerase. Both of they may be useful in the predicting of chemotherapeutic effect in ovarian cancer.