Culture filtrate of root endophytic fungus Piriformospora indica promotes the growth and lignan production of Linum album hairy root cultures

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• 作者：Vinod Kumar¹ ; Gaurav Rajauria² ; Vikram Sahai ; V.S. Bisaria ; ^{vbisaria@dbeb.iitd.ac.in} ; ^{vsbisaria@yahoo.com}
• 关键词：Linum album ; Piriformospora indica ; Podophyllotoxin ; 6-Methoxypodophyllotoxin ; Phenylalanine ammonia lyase
• 刊名：Process Biochemistry
• 出版年：2012
• 期刊代码：29_13595113
• 类别：bio
• 出版时间：June, 2012
• 卷：47
• 期：6
• 页码：901-907
• 文件大小：384 K

摘要

The effect of addition of autoclaved and filter-sterilized culture filtrate of Piriformospora indica (a root endophytic fungus) to the growing Linum album hairy root cultures on growth and lignan production was investigated. The addition resulted in a significant enhancement in lignan production and growth. The podophyllotoxin and 6-methoxypodophyllotoxin (the lignans) concentrations were maximally improved by 3.8 times (233.8 mg/L) and 4.4 times (131.9 mg/L) in comparison to control cultures, respectively, upon addition of 3.0%(v/v) filter-sterilized culture filtrate of P. indica to the hairy root cultures of L. album for exposure time of 48 h. This increase in the lignan content also coincided with the increase in phenylalanine ammonia lyase activity, which was 3.1-fold (371.4 渭kat/kg protein) higher compared to control cultures under the same conditions. The maximal increase in hairy root biomass was, however, obtained under different conditions; it was enhanced by 1.4 times (21.8 g/L) in comparison to control cultures, when 2%(v/v) filter-sterilized culture filtrate was in contact with L. album cultures for 96 h.