C/T conversion alters interleukin-1A promoter function in a human astrocyte cell line
- 作者：Xing Wei ; Xianming Chen ; Christine Fontanilla ; Liming Zhao ; Zhong Liang ; Richard Dodel ; Hampel Hampel ; Martin Farlow ; Yansheng Du
- 关键词：Alzheimer's disease ; Interleukin-1α ; Neuroinflammation ; Neurodegeneration ; Promoter polymorphism ; A-beta ; LPS
- 刊名：Life Sciences
- 出版年：2007
- 期刊代码：62_00243205
- 类别：imm
- 出版时间：27 February 2007
- 卷：80
- 期：12
- 页码：1152-1156
- 文件大小：202 K
摘要
Recently, association of an interleukin-1A promoter polymorphism (− 889, thymine/thymine (T/T)) with Alzheimer's disease was reported, suggesting that this cytokine may play an important role in disease development. To understand the mechanism underlying the interleukin-1A promoter's role in Alzheimer's disease, a study comparing promoter function of an interleukin-1A polymorphism was performed in the SVG astroglia cell line. The effects of thymine and cytosine on transcriptional activity of the interleukin-1A promoter were analyzed by testing luciferase-reporter activity in transfected SVG cells. Our results demonstrate that cytosine/thymine conversion increases activity of the interleukin-1A promoter in SVG cells. Both sodium salicylate and lovastatin are able to block induced promoter activities in astroglial cells. Induced promoter activity by the polymorphism (T/T) may result in the upregulation of interleukin-1α protein and “cytokine cycle” amplification, which may promote disease development.