Determination of limonin in rat plasma by liquid chromatography–electrospray mass spectrometry
- 作者：Yan Liang ; Lin Xie ; Xiao-Dong Liu ; Tong Lu ; Guang-Ji Wang and Yu-Zhu Hu
- 关键词：Limonin ; LC/ESI/MS ; Rat plasma
- 刊名：Journal of Pharmaceutical and Biomedical Analysis
- 出版年：2005
- 期刊代码：65_07317085
- 类别：ch
- 出版时间：2005
- 卷：39
- 期：5
- 页码：1031-1035
- 文件大小：226 K
摘要
A simple, sensitive and selective liquid chromatography coupled with electrospray ionization mass spectrometry (LC/ESI/MS) method for determination of limonin (LM) in rat plasma has been developed and validated. The method had advantages of a single liquid–liquid extraction with ether and high sensitivity. Analyses were conducted at a flow rate of 0.2 ml/min by a gradient elution. The detection utilized selected ion monitoring in the negative ion mode at m/z 460.00 and 423.15 for the deprotonated molecular ions of LM and the internal standard, respectively. The quantitation limit for LM in rat plasma was 1.0 ng/ml. The linearity was also excellent over the concentration range of 1.9–500 ng/ml of LM. The intra- and inter-day precision (relative standard deviation (R.S.D.%)) was lower than 10%and accuracy ranged from 90 to 110%, showing a good reproducibility. This developed method was successfully applied to analysis of LM in biological fluids.