Lack of significant influence for Fc纬RIIa-RH131 or hemoglobin AA/AS polymorphisms on immunity and susceptibility to uncomplicated malaria and existence of marked linkage between the two polymorphisms in Daraweesh
- 作者：Hayder A. Gihaa ; b ; gehaha2002@yahoo.com ; hayderag@agu.edu.bh ; Amre Nasrc ; d ; Nnaemeka C. Iriemename ; Marita Troye-Blombergf ; Klavs Berzinsf ; Gehad ElGhazalib ; g
- 关键词：Malaria susceptibility ; Polymorphism ; Fc纬RIIa-RH131 ; HbAS ; Linkage disequilibrium ; Sudan
- 刊名：Microbes and Infection
- 出版年：2012
- 期刊代码：65_12864579
- 类别：imm
- 出版时间：June, 2012
- 卷：14
- 期：6
- 页码：537-544
- 文件大小：175 K
摘要
Malaria signature on human genome is marked by several gene polymorphisms. HemoglobinAS (HbAS) is known to protect against severe malaria, but barely proved to protect against uncomplicated malaria (UM). Similarly, the influence of Fc纬RIIa-RH131 polymorphism on malaria is controversial. Polymorphisms in both genes were examined and levels of IgG subclasses against four malaria antigens were measured for 250 Fulani鈥檚 from Daraweesh, eastern Sudan. Morbidity data for up to nine years was available for 214 donors. Number of malaria episodes experienced by each individual during the study period was used as indicator for susceptibility to UM. PCR and RFLP were used for donors DNA genotyping and ELISA for antibodies measurement. Results revealed that neither Fc纬RIIa-RH131 alleles/genotypes nor HbAA/AS was significantly associated with malaria morbidity or with levels of IgG to test antigens. Both polymorphisms were in Hardy-Weinberg Equilibrium, interestingly, there was strong association between the two polymorphisms (linkage disequilibrium - LD) with D鈥猜?聽0.89. The association between the two polymorphisms was confirmed by analysis of independent material from a neighboring village. In conclusion, in Daraweesh both Fc纬RIIa-RH131 and HbAA/AS genotypes, independently or together, were not major markers for UM susceptibility, however, marked LD was observed between the two polymorphisms.