Genetic linkage maps of Dendrobium moniliforme and D. officinale based on EST-SSR, SRAP, ISSR and RAPD markers

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• 作者：Jiang-Jie Lu^a ; ^b ; ¹ ; ^{grantlujiang@hotmail.com} ; Hong-Yan Zhao^a ; ¹ ; ^{zhaohy1000@163.com} ; Na-Na Suo^a ; ^{584286349@qq.com} ; Shang Wang^a ; ^{476221771@qq.com} ; Bo Shen^a ; ^{bshen65@hzcnc.com} ; Hui-Zhong Wang^a ; ^{whz62@163.com} ; Jun-Jun Liu^a ; ^{liujuju@hotmail.com}
• 关键词：Dendrobium ; Double pseudo-testcross ; EST-SSR ; Genetic linkage ; ISSR ; SRAP
• 刊名：Scientia Horticulturae
• 出版年：2012
• 期刊代码：93_03044238
• 类别：abs
• 出版时间：1 April, 2012
• 卷：137
• 期：Complete
• 页码：1-10
• 文件大小：467 K

摘要

The genome organization of Dendrobium species remains largely unexplored. An F₁ family derived from the controlled interspecific hybridization between Dendrobium moniliforme and D. officinale were used to create genetic linkage maps of parental Dendrobiums using double pseudo-testcross strategy. A total of 422 markers, including 66 EST-SSR, 126 SRAP, 74 ISSR, and 156 RAPD markers, showed expected Mendelian segregation ratio and were used for genetic map construction. Genetic linkage groups with Kosambi genetic distances were constructed using a LOD score 鈮?#xA0;4 and a recombination threshold of 0.4. The map of D. moniliforme was 1127.9 cM in total length with 165 marker loci distributed in 17 linkage groups; and the D. officinale map consisted of 19 genetic linkage groups with a total length of 1210.9 cM positioned by 169 marker loci. The genetic maps constructed in this study can serve as a reference linkage map of Dendrobium species for efficient studies of comparative genetics, genetic mapping of traits with economic interest, and marker-assisted selection in horticultural breeding.