A direct and fast method to monitor lipid oxidation progress in model fatty acid methyl esters by high-performance size-exclusion chromatography
- 作者：G. Má ; rquez-Ruiz ; F. Holgado ; M.C. Garcí ; a-Martí ; nez ; M.C. Dobarganes
- 关键词：High-performance size-exclusion chromatography ; Lipid oxidation ; FAME ; Methyl linoleate ; Oxidation kinetics ; Tocopherol
- 刊名：Journal of Chromatography A
- 出版年：2007
- 期刊代码：47_00219673
- 类别：ch
- 出版时间：21 September 2007
- 卷：1165
- 期：1-2
- 页码：122-127
- 文件大小：377 K
摘要
A new method based on high-performance size-exclusion chromatography (HPSEC) is proposed to quantitate primary and secondary oxidation compounds in model fatty acid methyl esters (FAMEs). The method consists on simply injecting an aliquot sample in HPSEC, without preliminary isolation procedures neither addition of standard internal. Four groups of compounds can be quantified, namely, unoxidised FAME, oxidised FAME monomers including hydroperoxides, FAME dimers and FAME polymers. Results showed high repeatability and sensitivity, and substantial advantages versus determination of residual substrate by gas–liquid chromatography. Applicability of the method is shown through selected data obtained by numerous oxidation experiments on pure FAME, mainly methyl linoleate, at ambient and moderate temperatures.