Common pathways for receptor-mediated ingestion of Escherichia coli and LDL cholesterol by Entamoeba histolytica regulated in part by transmembrane kinase 39

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• 作者：Nathaniel C.V. Christy^a ; Sarah N. Buss^a ; ¹ ; William A. Petri Jr.^b ; ^{wap3g@virginia.edu}
• 关键词：Entamoeba histolytica ; Transmembrane kinase ; Phagocytosis ; Bacteria ; LDL receptor ; Cholesterol
• 刊名：International Journal for Parasitology
• 出版年：2012
• 期刊代码：124_00207519
• 类别：med
• 出版时间：April, 2012
• 卷：42
• 期：4
• 页码：393-400
• 文件大小：1625 K

摘要

The single-celled parasite, Entamoeba histolytica, is an enteric pathogen that ingests bacteria and host cells. Inhibition of phagocytosis renders the parasite avirulent. The ligand/receptor interactions that allow E. histolytica to phagocytose are not well understood. We hypothesised that E. histolytica trophozoites might accomplish ingestion through the utilisation of a scavenger receptor for cholesterol. Here we show that acetylated low density lipoprotein cholesterol was phagocytosed by amoebae via receptor-mediated mechanisms. Acetylated low density lipoprotein cholesterol competitively inhibited by 31 卤 1.3%(P < 0.005) the ingestion of Escherichia coli, but not erythrocytes and Jurkat T lymphocytes, suggesting a partially redundant phagocytic pathway for E. coli and cholesterol. Inducible expression of a signalling-dead dominant-negative version of E. histolytica transmembrane kinase 39 inhibited ingestion of E. coli by 55 卤 3%(P < 0.005) but not LDL particles. We concluded that ingestion of E. coli was regulated by TMK39 and partially shared the acetylated low density lipoprotein cholesterol uptake pathway.