Development of an oxidative dehydrogenation-based fluorescent probe for Cu²⁺ and its biological imaging in living cells

详细信息	查看全文 | 推荐本文 |

• 作者：Jiangli Fan ; ^{fanjl@dlut.edu.cn} ; Xiaojian Liu ; Mingming Hu ; Hao Zhu ; Fengling Song ; Xiaojun Peng ; ^{pengxj@dlut.edu.cn}
• 关键词：BODIPY ; Fluorescent probe ; Cu2+ ; Oxidative dehydrogenation ; Living cells
• 刊名：Analytica Chimica Acta
• 出版年：2012
• 期刊代码：2_00032670
• 类别：ch
• 出版时间：20 July, 2012
• 卷：735
• 期：Complete
• 页码：107-113
• 文件大小：1188 K

摘要

Based on a boron dipyrromethene (BODIPY) derivative containing an N, O and S tridentate ligand, a Cu²⁺ fluorescent probe BTCu was developed. The detection mechanism was verified as Cu²⁺-promoted oxidative dehydrogenation of an amine moiety, leading to a formation of a fluorescent Cu⁺-Schiff base complex. Free BTCu exhibited a maximum absorption wavelength at 496 nm, and a very weak maximum emission at 511 nm. Upon addition of various metals ions, it showed large fluorescence enhancement toward Cu²⁺ (417-fold in MeCN and 103-fold in MeCN/HEPES solution, respectively) with high selectivity. The detection limits are as low as 1.74 脳 10^鈭? M and 4.96 脳 10^鈭? M in the two different solutions, respectively. And BTCu could work in a wide pH range with an extraordinary low pK_a of 1.21 卤 0.06. Using fluorescence microscopy, the probe was shown to be capable of penetrating into living cells and imaging intracellular Cu²⁺ changes.