A rhodamine chromene-based turn-on fluorescence probe for selectively imaging Cu²⁺ in living cell

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• 作者：Wei-Yong Liu^a ; ¹ ; Hai-Ying Li^b ; ¹ ; Hong-Shui Lv^a ; Bao-Xiang Zhao^a ; ^{bxzhao@sdu.edu.cn} ; Jun-Ying Miao^b ; ^{miaojy@sdu.edu.cn}
• 关键词：Rhodamine chromene ; X-ray ; Fluorescence probe ; Copper ion ; Living cells
• 刊名：Spectrochimica Acta Part A ; Molecular and Biomolecular Spectroscopy
• 出版年：2012
• 期刊代码：99_13861425
• 类别：ch
• 出版时间：September, 2012
• 卷：95
• 期：Complete
• 页码：658-663
• 文件大小：1169 K

摘要

We describe the development of a rhodamine chromene-based turn-on fluorescence probe to monitor the intracellular Cu²⁺ level in living cells. The new fluorescent probe with a chlorine group in chromene moiety exhibits good membrane-permeable property than previous reported because the predicted lipophilicity of present probe 4 is stronger than that of methoxyl substituted probe in our previous work (CLogP of 4: 8.313, CLogP of methoxyl substituted probe: 7.706), and a fluorescence response toward Cu²⁺ under physiological conditions with high sensitivity and selectivity, and facilitates naked-eye detection of Cu²⁺. The fluorescence intensity was remarkably increased upon the addition of Cu²⁺ within 1 or 2 min, while the other sixteen metal ions caused no significant effect.