Enzymatic hydrolysis of lignocellulosic biomass in ionic liquid media for fermentable sugar production

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• 作者：Chia-Hung Su^a ; ^{chsu@mail.mcut.edu.tw} ; Ming-Hua Chung^a ; Hsin-Ju Hsieh^b ; Yu-Kaung Chang^a ; Jin-Chau Ding^a ; Hui-Ming Wu^c
• 关键词：Hydrolysis ; Sugarcane bagasse ; Cellulase ; Ionic liquid ; Glucose
• 刊名：Journal of the Taiwan Institute of Chemical Engineers
• 出版年：2012
• 期刊代码：P31_18761070
• 类别：ce
• 出版时间：July, 2012
• 卷：43
• 期：4
• 页码：573-577
• 文件大小：311 K

摘要

This study investigated the enzymatic in situ hydrolysis of sugarcane bagasse by Accellerase鈩?1500, a commercial cellulase, in the presence of 1-ethyl-3-methylimidazolium diethylphosphate (EMIM-DEP), which is a cellulose-dissolving solvent. The sugarcane bagasse was first delignified, and the remaining cellulose was used as substrate for subsequent enzymatic hydrolysis. The EMIM-DEP pretreatment and related enzyme-catalyzed conditions affecting the yield of glucose liberated from the delignified bagasse were optimized. The maximal hydrolysis yield of 82.86%was obtained at an EMIM-DEP concentration of 5%(v/v), pretreatment temperature of 50 掳C, pretreatment time of 3 h, enzyme-catalyzed reaction temperature of 40 掳C, and enzyme loading of 178.5 U/g-substrate.