Straighten up and fly right—microtubule dynamics and organization of non-centrosomal arrays in higher plants
- 作者：David W Ehrhardt
- 关键词：Bacterial strain ; Growth phase ; Intestinal epithelium ; Multidrug resistance
- 刊名：Current Opinion in Cell Biology
- 出版年：2008
- 期刊代码：58_09550674
- 类别：bio
- 出版时间：February 2008
- 卷：20
- 期：1
- 页码：107-116
- 文件大小：530 K
摘要
Salmonella Typhimurium DT104 is a zoonotic enteropathogen of increasing concern for human health. In this study, the influence of growth phase on invasiveness of a S. Typhimurium DT104 field isolate and two reference strains (SL1344 and ATCC 14028) was compared in IPEC J2 cells and mucosal explants from porcine ileum. Internalized bacteria were quantified by a gentamicin resistance assay. After 90 min of exposure to the apical aspect of epithelial monolayers or luminal surface of explants, internalization of all S. Typhimurium strains in mid-logarithmic phase of bacterial growth was comparable. Internalization of stationary phase bacteria was reduced relative to log phase bacteria, with DT104 exhibiting the greatest decrease. Growth phase-related differences in S. Typhimurium invasion are similar in porcine intestinal epithelial cells and mucosal explants, but may be greater in multidrug-resistant strains.