Expression of expansin genes during postharvest lignification and softening of ‘Luoyangqing’ and ‘Baisha’ loquat fruit under different storage conditions
- 作者：Shaolan Yang ; Chongde Sun ; Ping Wang ; Lanlan Shan ; Chong Cai ; Bo Zhang ; Wangshu Zhang ; Xian Li ; Ian Ferguson ; Kunsong Chen
- 关键词：Chilling injury ; Expansin ; Lignification ; Loquat fruit ; LTC ; 1-MCP ; Softening
- 刊名：Postharvest Biology and Technology
- 出版年：2008
- 期刊代码：89_09255214
- 类别：abs
- 出版时间：July 2008
- 卷：49
- 期：1
- 页码：46-53
- 文件大小：762 K
摘要
Four expansin cDNA fragments, EjEXPA1, EjEXPA2, EjEXPA3 and EjEXPA4, were isolated and characterized from loquat (Eriobotrya japonica Lindl.) fruit. EjEXPA1 mRNA accumulated consistently with the increase in fruit firmness in 0 °C storage of ‘Luoyangqing’ (LYQ) fruit, where chilling injury with increased fruit firmness due to lignification was observed. EjEXPA1 mRNA levels were lower in fruit that underwent low temperature conditioning (LTC, 6 d at 5 °C then 4 d at 0 °C), and in 1-methylcyclopropene (1-MCP) treated fruit, in both cases where chilling injury was alleviated. Fruit of the ‘Baisha’ (BS) cultivar soften after harvest rather than increase in firmness, and high expression levels of EjEXPA1 and EjEXPA4 accompanied the softening of BS fruit stored at 20 °C; such mRNA accumulation was much lower when fruit were stored at 0 °C, where softening was significantly inhibited by the low temperature. Very low expression of EjEXPA2 and EjEXPA3 was observed during storage of both LYQ and BS fruit under the different storage conditions. Our results showed that of the four genes characterized, EjEXPA1 might be associated with chilling-induced lignification while both EjEXPA1 and EjEXPA4 were closely related to softening of loquat fruit during the postharvest period.