The surface-localised 伪-enolase of Mycoplasma suis is an adhesion protein

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• 作者：Sabrina A. Schreiner^b ; ¹ ; Albina Sokoli^a ; ^c ; ¹ ; Kathrin M. Felder^a ; ^c ; Max M. Wittenbrink^b ; Sarah Schwarzenbach^b ; Bruno Guhl^c ; Katharina Hoelzle^b ; Ludwig E. Hoelzle^a ; ^{ludwig.hoelzle@uni-hohenheim.de}
• 关键词：Haemotrophic mycoplasmas ; Alpha-enolase ; Adhesion ; Erythrocytes
• 刊名：Veterinary Microbiology
• 出版年：2012
• 期刊代码：106_03781135
• 类别：abs
• 出版时间：23 April, 2012
• 卷：156
• 期：1-2
• 页码：88-95
• 文件大小：593 K

摘要

Mycoplasma suis belongs to the haemotrophic mycoplasmas which colonise red blood cells of a wide range of vertebrates. Adhesion to red blood cells is the crucial step in the unique lifecycle of M. suis. Due to the lack of a cultivation system, identification of adhesion structures has been difficult. So far, only one adhesion protein, i.e. MSG1 was identified. In order to determine further adhesion molecules of M. suis, we screened genomic M. suis libraries and performed Southern blot hybridisation analyses of genomic M. suis DNA. The 伪-enolase of M. suis was identified and analysed genetically and functionally. The encoding gene has 1623 bp in size. The deduced amino acid sequence showed an overall identity of 59.6-65.1%to 伪-enolases of other pathogenic mycoplasmas. The 540 aa M. suis 伪-enolase displays a size extension of about 90 aa in comparison to 伪-enolases of other mycoplasmas. Recombinant 伪-enolase expressed in Escherichia coli demonstrated immunogenicity in experimentally infected pigs. Immunoblot, confocal laser scanning microscopy and immune electron microscopy analysis using antibodies against recombinant 伪-enolase, indicate the membrane and surface localisation of native 伪-enolase in M. suis, though no typical signal sequences exist. Furthermore, we showed that recombinant 伪-enolase binds to porcine erythrocyte lysate in a dose-dependent manner. E. coli transformants which express 伪-enolase on their surface acquire the ability to adhere to porcine red blood cells. In conclusion, our observations indicate that 伪-enolase could be involved in the adhesion of M. suis to porcine red blood cells.