Ectopic bone formation by 3D porous calcium phosphate-Ti6Al4V hybrids produced by perfusion electrodeposition

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• 作者：Yoke Chin Chai^a ; ^b ; ^c ; ¹ ; ^{yokechin.chai@med.kuleuven.be} ; Greet Kerckhofs^c ; ^g ; ² ; ^{greet.kerckhofs@mtm.kuleuven.be} ; Scott J. Roberts^a ; ^c ; ¹ ; ^{Scott.Roberts@med.kuleuven.be} ; Simon Van Bael^c ; ^d ; ^e ; ³ ; ^{Simon.VanBael@mech.kuleuven.be} ; Evert Schepers^c ; ^f ; ⁴ ; ^{evert.schepers@med.kuleuven.be} ; Jozef Vleugels^g ; ⁵ ; ^{jozef.vleugels@mtm.kuleuven.be} ; Frank P. Luyten^a ; ^c ; ¹ ; ^{Frank.Luyten@uz.kuleuven.be} ; Jan Schrooten^c ; ^g ; ^{Jan.Schrooten@mtm.kuleuven.be}
• 关键词：Bone formation ; Calcium phosphate ; Osteoinduction ; Electrodeposition ; Titanium scaffolds
• 刊名：Biomaterials
• 出版年：2012
• 期刊代码：7_01429612
• 类别：ms
• 出版时间：June, 2012
• 卷：33
• 期：16
• 页码：4044-4058
• 文件大小：4596 K

摘要

Successful clinical repair of non-healing skeletal defects requires the use of bone substitutes with robust bone inductivity and excellent biomechanical stability. Thus, three-dimensionally functionalised porous calcium phosphate-Ti6Al4V (CaP-Ti) hybrids were produced by perfusion electrodeposition, and the in聽vitro and in聽vivo biological performances were evaluated using human periosteum derived cells (hPDCs). By applying various current densities at the optimised deposition conditions, CaP coatings with sub-micrometer to nano-scale porous crystalline structures and different ion dissolution kinetics were deposited on the porous Ti6Al4V scaffolds. These distinctive physicochemical properties caused a significant impact on in聽vitro proliferation, osteogenic differentiation, and matrix mineralisation of hPDCs. This includes a potential role of hPDCs in mediating osteoclastogenesis for the resorption of CaP coatings, as indicated by a significant down-regulation of osteoprotegerin (OPG) gene expression and by the histological observation of abundant multi-nucleated giant cells near to the coatings. By subcutaneous implantation, the produced hybrids induced ectopic bone formation, which was highly dependent on the physicochemical properties of the CaP coating (including the Ca²⁺ dissolution kinetics and coating surface topography), in a cell density-dependent manner. This study provided further insight on stem cell-CaP biomaterial interactions, and the feasibility to produced bone reparative units that are predictively osteoinductive in聽vivo by perfusion electrodeposition technology.