Measurement of lysophospholipase D/autotaxin activity in human serum samples

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• 作者：Kazuhiro Nakamura ; Ryunosuke Ohkawa ; Shigeo Okubo ; Minoru Tozuka ; Mitsumasa Okada ; Shinya Aoki ; Junken Aoki ; Hiroyuki Arai ; Hitoshi Ikeda ; Yutaka Yatomi
• 关键词：Lysophospholipase D ; Autotaxin ; Lysophosphatidic acid ; Lysophosphatidylcholine ; Plasma
• 刊名：Clinical Biochemistry
• 出版年：2007
• 期刊代码：55_00099120
• 类别：med
• 出版时间：February 2007
• 卷：40
• 期：3-4
• 页码：274-277
• 文件大小：130 K

摘要

>Objectives:

Lysophospholipase D (lysoPLD)/autotaxin regulates the blood levels of lysophosphatidic acid (LPA), and we designed a serum lysoPLD assay for use in clinical laboratory testing.

Design and methods:

LysoPLD activity was assessed based on the amount of choline released with lysophosphatidylcholine as the substrate.

Results and conclusions:

It was confirmed that the lysoPLD assay can be applied to clinical laboratory testing. The serum lysoPLD activity in healthy female subjects was significantly higher than that in the male subjects.