Characterisation and trophic functions of murine embryonic macrophages based upon the use of a Csf1r–EGFP transgene reporter
- 作者：Fiona Rae ; Kyra Woods ; Tedjo Sasmono ; Naomi Campanale ; Darrin Taylor ; Dmitry A. Ovchinnikov ; Sean M. Grimmond ; David A. Hume ; Sharon D. Ricardo ; Melissa H. Little
- 关键词：Embryonic macrophage ; Expression profiling ; Colony-stimulating factor 1 (CSF-1) ; Csf1R ; c-fms ; Kidney development ; Organogenesis ; EGFP transgene ; Alternate macrophage activation
- 刊名：Developmental Biology
- 出版年：2007
- 期刊代码：167_00121606
- 类别：bio
- 出版时间：1 August 2007
- 卷：308
- 期：1
- 页码：232-246
- 文件大小：5263 K
摘要
All solid organs contain resident monocyte-derived cells that appear early in organogenesis and persist throughout life. These cells are critical for normal development in some organs. Here we report the use of a previously described transgenic line, with EGFP driven by the macrophage-restricted Csf1r (c-fms) promoter, to image macrophage production and infiltration accompanying organogenesis in many tissues. Using microarray analysis of FACS-isolated EGFP-positive cells, we show that fetal kidney, lung and brain macrophages show similar gene expression profiles irrespective of their tissue of origin. EGFP-positive cells appeared in the renal interstitium from 12 days post coitum, prior to nephrogenesis, and maintain a close apposition to renal tubules postnatally. CSF-1 added to embryonic kidney explants increased overall renal growth and ureteric bud branching. Expression profiling of tissue macrophages and of CSF-1-treated explants showed evidence of the alternate, pro-proliferative (M2) activation profile, including expression of macrophage mannose receptor (CD206), macrophage scavenger receptor 2 (Msr2), C1q, CD163, selenoprotein P, CCL24 and TREM2. This response has been associated with the trophic role of tumour-associated macrophages. These findings suggest a trophic role of macrophages in embryonic kidney development, which may continue to play a similar role in postnatal repair.