Relayed magnetization transfer from nuclear Overhauser effect and chemical exchange observed by in vivo ³¹P MRS in rat brain

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• 作者：Fei Du^a ; ^b ; ^c ; ^{fdu@mclean.harvard.edu} ; Yi Zhang^a ; Wei Chen^a
• 关键词：In vivo 31P MRS ; Spin diffusion ; Magnetization transfer ; Chemical exchange ; Nuclear Overhauser effect (NOE)
• 刊名：Magnetic Resonance Imaging
• 出版年：2012
• 期刊代码：188_0730725x
• 类别：med
• 出版时间：June, 2012
• 卷：30
• 期：5
• 页码：716-721
• 文件大小：623 K

摘要

The ³¹P magnetization transfer effects among nuclear magnetic resonances (NMRs) of phosphocreatine (PCr), 纬-adenosine-5'-triphosphate (纬-ATP) and inorganic phosphate (Pi) have been attributed to the chemical exchange reactions among PCr, ATP and Pi catalyzed by creatine kinase (CK) and ATPase enzymes and, therefore, are commonly applied in situ to measure chemical exchange fluxes involving two chemically coupled CK and ATPase reactions (i.e., PCr鈫擜TP鈫擯i) by selectively saturating 纬-ATP resonance. Besides the expected reductions in the Pi and PCr NMR signals upon saturating 纬-ATP resonance, one particularly interesting phenomenon showing decreases in 伪-ATP and 尾-ATP signals was also observed. The underlying mechanism was investigated and identified via saturating NMR of 尾-ATP in the present study. The unique relayed magnetization transfer effects through spin diffusion were observed in the rat brain using in vivo ³¹P magnetic resonance spectroscopy.