Analysis of magnolol and honokiol in biological fluids by capillary zone electrophoresis

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• 作者：Chiu-Ling Chen ; Po-Ling Chang ; Shoei-Sheng Lee ; Fu-Chuo Peng ; Ching-Hua Kuo ; Huan-Tsung Chang
• 关键词：Magnolol ; Honokiol ; Capillary zone electrophoresis ; Laser-induced fluorescence detection ; Biological fluids
• 刊名：Journal of Chromatography A
• 出版年：2007
• 期刊代码：47_00219673
• 类别：ch
• 出版时间：23 February 2007
• 卷：1142
• 期：2
• 页码：240-244
• 文件大小：160 K

摘要

Capillary zone electrophoresis (CZE) method was used for analysis of magnolol and honokiol. Under the optimized condition, CZE with UV absorption detection provided that the limit of detection was at μM level. To enhance detection sensitivity of magnolol and honokiol, CZE separation system was coupled with a laser-induced fluorescence (LIF) detector for the first time. The limits of detection of magnolol and honokiol were 12 nM (3.20 ng ml⁻¹) and 18 nM (4.79 ng ml⁻¹), respectively, showing that the CZE-LIF system provides greater than 100-fold sensitivity improvements than does the CZE-UV system. The developed method was applied to analyze magnolol and honokiol in spiked human plasma samples, microsome incubation samples as a preliminary demonstration of its potential in pharmacokinetic studies.