UGT75L6 and UGT94E5 mediate sequential glucosylation of crocetin to crocin in Gardenia jasminoides

详细信息	查看全文 | 推荐本文 |

• 作者：Mai Nagatoshi^a ; Kazuyoshi Terasaka^a ; Miki Owaki^a ; Makiko Sota^a ; Tatsunori Inukai^a ; Akito Nagatsu^b ; Hajime Mizukami^a ; ^{hajimem@phar.nagoya-cu.ac.jp}
• 关键词：PSPG ; plant secondary product glycosyltransferase
• 刊名：FEBS Letters
• 出版年：2012
• 期刊代码：70_00145793
• 类别：bio
• 出版时间：5 April, 2012
• 卷：586
• 期：7
• 页码：1055-1061
• 文件大小：923 K

摘要

Crocin is an apocarotenoid glycosyl ester accumulating in fruits of Gardenia jasminoides and used as a food coloring and nutraceutical. For the first time, the two glucosyltransferases UGT75L6 and UGT94E5 that sequentially mediate the final glucosylation steps in crocin biosynthesis in G. jasminoides have been identified and functionally characterized. UGT75L6 preferentially glucosylates the carboxyl group of crocetin yielding crocetin glucosyl esters, while UGT94E5 glucosylates the 6鈥?hydroxyl group of the glucose moiety of crocetin glucosyl esters. The expression pattern of neither UGT75L6 nor UGT94E5 correlated with the pattern of crocin accumulation in G. jasminoides.