Atmospheric pressure visible-wavelength MALDI-MS
- 作者：Zhen Sun ; Eric W. Findsen ; Dragan Isailovic ; Dragan.Isailovic@utoledo.edu
- 关键词：Atmospheric pressure ; Visible-wavelength MALDI-MS ; Dye ; Peptide ; Oligosaccharide ; Synthetic polymer
- 刊名：International Journal of Mass Spectrometry
- 出版年：2012
- 期刊代码：43_13873806
- 类别：ch
- 出版时间：1 April, 2012
- 卷：315
- 期：Complete
- 页码：66-73
- 文件大小：1049 K
摘要
An atmospheric pressure (AP) visible-wavelength MALDI source was developed and coupled to a quadrupole time-of-flight (Q-TOF) mass spectrometer. This instrument employed a pulsed laser emitting light at a wavelength of 532 nm to desorb and ionize samples such as dyes, oligosaccharides, peptides, and synthetic polymers at atmospheric pressure. Ions formed were analyzed by the Q-TOF in both MS and tandem MS (MS/MS) modes. Several visible-wavelength absorbing dyes were successfully utilized and studied as matrices. Among these dyes, nuclear fast red (NFR), rhodamine B isothiocyanate (RITC), and 5(6)-carboxytetramethyl-rhodamine N-succinimidyl ester (TAMRA, SE) are reported as effective visible-wavelength MALDI-MS matrices for the first time. This report demonstrates applicability of visible-wavelength AP MALDI-MS and AP MALDI MS/MS to the detection and structural analysis of dye molecules, biomolecules, and synthetic polymers.