Production of biofuels from pretreated microalgae biomass by anaerobic fermentation with immobilized Clostridium acetobutylicum cells
- 作者：E.N. Efremenko ; elena_efremenko@list.ru ; A.B. Nikolskaya ; I.V. Lyagin ; O.V. Senko ; T.A. Makhlis ; N.A. Stepanov ; O.V. Maslova ; F. Mamedova ; S.D. Varfolomeev
- 关键词：ABE fermentation ; acetone&ndash ; butanol&ndash ; ethanol fermentation ; PVA ; poly(vinyl alcohol) ; DMSO ; dimethyl sulfoxide ; SDS ; sodium dodecyl sulfate
- 刊名：Bioresource Technology
- 出版年：2012
- 期刊代码：11_09608524
- 类别：ce
- 出版时间：June, 2012
- 卷：114
- 期：Complete
- 页码：342-348
- 文件大小：301 K
摘要
The purpose of this work was to study the possible use of pretreated biomass of various microalgae and cyanobacteria as substrates for acetone-butanol-ethanol (ABE) fermentation by Clostridium acetobutylicum cells immobilized into poly(vinyl alcohol) cryogel. To this end, the biochemical composition of photosynthetic microorganisms cultivated under various conditions was studied. The most efficient technique for pretreating microalgal biomass for its subsequent conversion into biofuels appeared to be thermal decomposition at 108 掳C. For the first time the maximum productivity of the ABE fermentation in terms of hydrogen (8.5 mmol/L medium/day) was obtained using pretreated biomass of Nannochloropsis sp. Maximum yields of butanol and ethanol were observed with Arthrospira platensis biomass used as the substrate. Immobilized Clostridium cells were demonstrated to be suitable for multiple reuses (for a minimum of five cycles) in ABE fermentation for producing biofuels from pretreated microalgal biomass.