Decalpenic acid induces early osteoblastic markers in pluripotent mesenchymal cells via activation of retinoic acid receptor 纬

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• 作者：Shuichi Sakamoto^a ; ^b ; ^{sakamotos@bikaken.or.jp} ; Fukiko Kojima^a ; Isao Momose^b ; Manabu Kawada^b ; Hayamitsu Adachi^a ; Yoshio Nishimura^a
• 关键词：MSC ; mesenchymal stem cell ; BMP ; bone morphogenetic protein ; RARE ; retinoic acid response element ; DPA ; decalpenic acid ; ATRA ; all-trans retinoic acid ; RA ; retinoic acid ; PM ; purmorphamine ; RAR ; retinoic acid receptor ; RXR ; rexinoid receptor ; LBD ; ligand
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2012
• 期刊代码：159_0006291x
• 类别：bio
• 出版时间：15 June, 2012
• 卷：422
• 期：4
• 页码：751-757
• 文件大小：689 K

摘要

Decalpenic acid is a natural small molecule previously isolated from the fermentation broth of fungi that induces early osteoblastic markers in pluripotent mesenchymal cells. Treatment of mouse pluripotent mesenchymal C3H10T1/2 cells with decalpenic acid gave rise to a morphological change similar to that induced by the treatment with retinoic acid, i.e. the cells adopted a more elongated spindle shape. Using a retinoic acid response element reporter and receptor activity assays, we show that decalpenic acid is a new retinoid with selectivity towards retinoic acid receptors 纬 and 伪. The induction of early osteoblastic markers by decalpenic acid was significantly inhibited by treatment with the retinoid antagonist, LE540, or with small interfering RNA-mediated knockdown of retinoic acid receptor 纬. These results demonstrated that decalpenic acid induces early osteoblastic markers in pluripotent mesenchymal cells through activation of retinoic acid receptor 纬.