Cytokine profiles using whole-blood assays can discriminate between tuberculosis patients and healthy endemic controls in a BCG-vaccinated population
- 作者：Hussain ; Rabia ; Kaleem ; Arnawaz ; Shahid ; Firdaus ; Dojki ; Maqboola ; Jamil ; Bushra ; Mehmood ; Hammad ; et. al.
- 关键词：Cytokines ; Whole-blood assay ; Tuberculosis ; Pulmonary and extrapulmonary
- 刊名：Journal of Immunological Methods
- 出版年：2002
- 期刊代码：57_00221759
- 类别：imm
- 出版时间：June 1, 2002
- 卷：264
- 期：1-2
- 页码：95-108
- 文件大小：273 K
摘要
Whole-blood assays (WB) provide a simple tool for assessing immune cytokine profiles which may be useful laboratory predictors of early disease, aiding the evaluation of new tuberculosis (TB) vaccines and offering insights into disease pathogenesis. Although BCG does not provide protection against pulmonary disease in TB endemic areas, it does modulate immune responses to mycobacterial antigens. It is important, therefore, to evaluate any new tool in an endemic setting in both BCG vaccinees and patients with tuberculosis. We have assessed the optimal conditions in terms of dose and kinetics of those cytokines which are released early (TNF-α, IL6 and TGF-β, IL10) or (interferon [IFN]-γ and IL5) in WB cultures stimulated with mitogens and mycobacterial antigens. Responses were studied in parallel in untreated TB patients and endemic control groups. Optimal responses to LPS (predominantly monocyte-derived) occurred on days 1–2, whereas for PHA (predominantly T-cell-derived), they were on days 3–5. Secreted Mycobacterium tuberculosis culture filtrate proteins (CFP) provided a stronger stimulus for monocyte-derived cytokines compared to PPD, but both antigens were comparable for induction of T-cell cytokines. Using unpaired Student's t-tests, pulmonary tuberculosis patients (P.TB; n