Purification and characterization of a chitosanase from Serratia marcescens TKU011

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• 作者：San-Lang Wang ; Jo-Hua Peng ; Tzu-Wen Liang ; Kao-Cheng Liu
• 关键词：Serratia marcescens ; Chitosanase ; Chitosan ; Chitin ; Shrimp shell wastes
• 刊名：Carbohydrate Research
• 出版年：2008
• 期刊代码：15_00086215
• 类别：ch
• 出版时间：9 June 2008
• 卷：343
• 期：8
• 页码：1316-1323
• 文件大小：166 K

摘要

A chitosanase was purified from the culture supernatant of Serratia marcescens TKU011 with shrimp shell wastes as the sole carbon/nitrogen source. Zymogram analysis revealed the presence of chitosanolytic activity corresponding to one protein, which was purified by a combination of ion-exchange and gel-filtration chromatography. The molecular weight of the chitosanase was 21 kDa and 18 kDa estimated by SDS–PAGE and gel-filtration, respectively. The optimum pH, optimum temperature, pH stability, and thermal stability of the chitosanase were 5, 50 °C, pH 4–8, and <50 °C, respectively. The chitosanase was inhibited completely by EDTA, Mn²⁺, and Fe²⁺. The results of peptide mass mapping showed that three tryptic peptides of the chitosanase were identical to a chitin-binding protein Cbp21 from S. marcescens (GenBank accession number gi58177632) with 63%sequence coverage.