Overexpression of Serratia marcescens lipase in Escherichia coli for efficient bioresolution of racemic ketoprofen
- 作者：Zhang-De Long ; Jian-He Xu ; Li-Li Zhao ; Jiang Pan ; Sheng Yang ; Ling Hua
- 关键词：Serratia marcescens lipase ; Chiral resolution ; Ketoprofen ; Surfactant ; Overexpression
- 刊名：Journal of Molecular Catalysis B ; Enzymatic
- 出版年：2007
- 期刊代码：55_13811177
- 类别：ce
- 出版时间：2 July 2007
- 卷：47
- 期：3-4
- 页码：105-110
- 文件大小：455 K
摘要
Lipase from Serratia marcescens ECU1010 was cloned and overexpressed in E. coli. After optimization, the maximum lipase activities reached 5000–6000 U/l and this recombinant lipase could enantioselectively hydrolyze (S)-ketoprofen esters into (S)-ketoprofen. Among six alkyl esters of racemic ketoprofen investigated, this lipase showed the best enantioselectivity for the kinetic resolution of ketoprofen ethyl ester, with an eep (enantiomeric excess of product) of 91.6%and E-value of 63 obtained at 48.2%conversion. Twelve nonionic surfactants were tested for enhancing the enantioselectivity of this lipase in the bioresolution of ketoprofen ethyl ester. A very high E-value of 1084 was achieved, with an optical purity of >99%eep and a yield of 42.6%in the presence of 3%Brij 92V. Further studies showed that the selectivity of the lipase was improved with the increase of Brij 92V concentration. The substrate (ketoprofen ethyl ester) does not inhibit the lipase activity, while the product (S)-ketoprofen inhibits the lipase activity to some extent. These results indicate that the S. marcescens lipase is very useful for biocatalytic production of chiral profens such as (S)-ketoprofen.