Amperometric detection of ascorbic acid in honey using ascorbate oxidase immobilised on amberlite IRA-743

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• 作者：Vané ; zia Liane da Silva ; Marcos Rodrigues Facchini Cerqueira ; Denise Lowinsohn ; Maria Auxiliadora Costa Matos ; Renato Camargo Matos ; ^{renato.matos@ufjf.edu.br}
• 关键词：Amperometry ; Honey ; Ascorbic acid
• 刊名：Food Chemistry
• 出版年：2012
• 期刊代码：48_03088146
• 类别：abs
• 出版时间：1 August, 2012
• 卷：133
• 期：3
• 页码：1050-1054
• 文件大小：375 K

摘要

A differential amperometric method for the specific determination of ascorbic acid in honey was developed by association of a flow injection analysis (FIA) system and a tubular reactor containing the ascorbate oxidase enzyme immobilised. A gold electrode modified by electrochemical deposition of palladium was employed as working electrode. Ascorbic acid was quantified in seven samples of commercial honeys using a potential of +0.60 V vs. Ag/AgCl_(sat). The linear dynamic range in ascorbic acid extends from 1 to 50 渭mol L^鈭?, at pH 7.0. At flow rate of 1.5 mL min^鈭? and injecting 250 渭L sample volumes, a sampling frequency of 180 determinations per hour is afforded. The detection and quantification limit of this method are 0.14 and 0.49 渭mol L^鈭?, respectively. The samples analyses were compared with the volumetric method, and showed an excellent correlation between the methods.