To analyse the role of CCR5 co-receptor density and proviral DNA load in the control of plasma HIV-viral load after HAART interruption, comparing patients whose plasma HIV load was persistently below 4 log10 RNA copies/mL, defined as “HIV controllers”, with patients who showed a viral load higher than 4 log10 RNA copies/mL, defined as “non-controllers”.
Proviral DNA load quantification (N = 55) and CCR5 co-receptor density (N = 29) were determined in HIV-infected patients on prolonged HAART interruption.
Twenty-three percent of our HAART interruption cohort were classified as HIV controllers, while 77%were classified as non-controllers. CCR5 co-receptor density was statistically higher in HIV controllers than in non-controllers, while proviral DNA load was not different between them. CCR5 co-receptor density in activated CD4 cells was independently associated with HIV plasma load after interruption.
The observation of a higher CCR5 co-receptor expression in HIV controllers suggests that HIV infection leads to the selection of CD4 cells with low CCR5 co-receptor density after HAART interruption.