Anti-dermatophytic activity of marine sponge, Sigmadocia carnosa (Dendy) on clinically isolated fungi

详细信息	查看全文 | 推荐本文 |

• 作者：NB Dhayanithi^a ; ^{microbiodhaya@gmail.com} ; TT Ajith Kumar^a ; M Kalaiselvam^a ; T Balasubramanian^a ; N Sivakumar^b
• 关键词：Dermatophytic fungi ; Sigmadocia Carnosa ; Anti-fungal activity ; Spore germination assay ; Qualitative analysis of ; Active compounds
• 刊名：Asian Pacific Journal of Tropical Biomedicine
• 出版年：2012
• 期刊代码：pca8_22211691
• 类别：bio
• 出版时间：August, 2012
• 卷：2
• 期：8
• 页码：635-639
• 文件大小：292 K

摘要

Objective

To screen the anti-fungal effects and find out the active metabolites from sponge, Sigmadocia carnosa (S. carnosa) against four dermatophytic fungi.

Methods

The methanol, ethyl acetate and acetone extract of marine sponge, S. carnosa was examined against Trichophyton mentagrophytes (T. mentagrophytes), Trichophyton rubrum (T. rubrum), Epidermophyton floccosum (E. floccosum) and Microsporum gypseum (M. gypseum) and qualitative analysed to find out the active molecules.

Results

The methanol extract of sponge was expressed significant activity than ethyl acetate and acetone. The minimum inhibitory concentration (MIC) of methanol extract of sponge that resulted in complete growth inhibition of T. mentagrophytes, T. rubrum, E. floccosum and M. gypseum were found to 125, 250, 250 and 250 渭g/mL respectively. But, 100%inhibition of fungal spore germination was observed in T. mentagrophytes at 500 渭g/mL concentration followed by T. rubrum, E. floccosum and M. gypseum at 1 000 渭g/mL concentration. Other two extracts showed weak anti spore germination activity against the tested dermatophytic fungi. Methanol extracts showed presence of terpenoids, steroids, alkaloids, saponins and glycosides.

Conclusion

Based on the literature, this is the first study which has conducted to inhibit the growth and spore germination of dermatophytic fungi with S. carnosa. Further research also needs to purify and characterize the secondary metabolites from the sponge, S. carnosa for the valuable source of novel substances for future drug discovery.