Biological activities of ACL-I and physicochemical properties of ACL-II, lectins isolated from the marine sponge Axinella corrugata

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• 作者：Roger R. Dresch^a ; ^{rrdresch@gmail.com} ; Clé ; a B. Lerner^b ; Beatriz Mothes^b ; Vera M.T. Trindade^c ; Amé ; lia T. Henriques^a ; Magdolna M. Vozá ; ri-Hampe^c
• 关键词：Axinella corrugata ; Cytotoxicity ; Lectin ; Marine sponge ; Mitogenicity
• 刊名：Comparative Biochemistry and Physiology ; Part B
• 出版年：2012
• 期刊代码：50_10964959
• 类别：bio
• 出版时间：April, 2012
• 卷：161
• 期：4
• 页码：365-370
• 文件大小：323 K

摘要

Lectin II from the marine sponge Axinella corrugata (ACL-II) was purified by affinity chromatography on rabbit erythrocytic stroma incorporated into a polyacrylamide gel, followed by gel filtration on Ultrogel AcA 44 column. Purified ACL-II is a lectin with an Mr of 80 kDa and 78 kDa, estimated by SDS-PAGE and by FPLC on Superose 12 HR column, respectively. ACL-II mainly agglutinates native rabbit erythrocytes and this hemagglutinating activity is independent of Ca^2 +, Mg^2 + and Mn^2 +, but is inhibited by d-galactose, chitin and N-acetyl derivatives, with the exception of GalNAc. ACL-II is stable for up to 65 掳C for 30 min, with a better stability at a pH range of 2 to 6. In contrast, ACL-I displays a strong mitogenic and cytotoxic effect.