Pre-hydrolysis improves utilisation of dietary protein in the larval teleost Atlantic halibut (Hippoglossus hippoglossus L.)

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• 作者：Sigurd K. Tonheim ; Marit Espe ; Kristin Hamre and Ivar Rø ; nnestad
• 关键词：Teleost fish larvae ; Protein ; Hydrolysate ; Amino acids ; Tube-feeding ; Digestion ; Absorption ; Assimilation ; Catabolism ; Faecal evacuations
• 刊名：Journal of Experimental Marine Biology and Ecology
• 出版年：2005
• 期刊代码：66_00220981
• 类别：abs
• 出版时间：2005
• 卷：321
• 期：1
• 页码：19-34
• 文件大小：234 K

摘要

A protein preparation labelled by incorporation of [U]¹⁴C-AA was hydrolysed to various degrees and administered to a teleost fish larva (Atlantic halibut, Hippoglossus hippoglossus L.) by tube-feeding, and its post-administration utilisation was studied. Three treatments were prepared: IntP—intact protein, PHP—pepsin-hydrolysed protein, and HHP—highly hydrolysed protein (using pepsin, trypsin, endoproteinase Glu-C, Asp-N, and Pro-C). At small doses (11.4±1.5 μg larvae⁻¹), the intact protein (IntP) was digested and absorbed to 36±5.5%. However, the relative absorption efficiency of the intact protein was reduced as the dose increased. Absorption efficiency was higher when the protein was hydrolysed prior to feeding the larvae and was constant at 63%(R²