Association between DNA strand breakage and oxidative, inflammatory and endothelial biomarkers in type 2 diabetes

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• 作者：Etiane Tatsch^a ; ^b ; Guilherme V. Bochi^a ; ^c ; Sí ; lvia J. Piva^b ; José ; A.M. De Carvalho^a ; ^b ; Helena Kober^a ; ^c ; Vanessa D. Torbitz^a ; Thiago Duarte^a ; Cristiane Signor^d ; Adriane C. Coelho^e ; Marta M.M.F. Duarte^f ; Greice F.F.S. Montagner^d ; Ivana B.M. Da Cruz^c ; ^d ; Rafael N. Moresco^a ; ^b ; ^c ; ^{rnmoresco@yahoo.com.br}
• 关键词：ROS ; reactive oxygen species ; NADPH ; nicotinamide adenine dinucleotide phosphatase ; AGEs ; advanced glycation end products ; NO ; nitric oxide ; IL-6 ; interleukin-6 ; BMI ; body mass index ; HbA1c ; glycated hemoglobin ; NOx ; nitrate/nitrite ; PBS ; phosphate buffer
• 刊名：Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis
• 出版年：2012
• 期刊代码：115_00275107
• 类别：bio
• 出版时间：1 April, 2012
• 卷：732
• 期：1-2
• 页码：16-20
• 文件大小：368 K

摘要

Evidence has been presented recently that type 2 diabetes patients have an increased level of DNA damage. This DNA damage could be associated with oxidative, inflammatory, and endothelial biomarkers and could represent a possible indication of injury in the endothelium and induction of inflammation in type 2 diabetes. To confirm this possible association, DNA strand breakage was evaluated by use of the comet assay and its association with oxidative, inflammatory, and endothelial biomarkers in type 2 diabetes patients. A case-control study (30 healthy controls and 32 subjects with type 2 diabetes) was performed to evaluate the association between DNA damage and NOx (nitrate/nitrite), interleukin-6 (IL-6), urinary albumin, fasting glucose, and glycated hemoglobin (HbA_1c) levels. Type 2 diabetes patients presented higher DNA damage than control subjects, higher levels of IL-6 and urinary albumin, and lower NOx. Significant correlations between DNA damage and NOx (r = 鈭?.303, p = 0.016), IL-6 (r = 0.845, p < 0.001), urinary albumin (r = 0.496, p < 0.001), fasting glucose (r = 0.449, p < 0.001), and HbA_1c (r = 0.575, p < 0.001) were reported. Our findings showed an increase of DNA damage in type 2 diabetes especially in those patients with poor glycemic control and associations among NOx, IL-6 and urinary albumin levels with DNA damage.