Synthesis of ¹³C- and ¹⁴C-labeled dinucleotide mRNA cap analogues for structural and biochemical studies

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• 作者：Karolina Piecyk^a ; Richard E. Davis^b ; Marzena Jankowska-Anyszka^a ; ^{marzena@chem.uw.edu.pl}
• 关键词：Cap analogue ; TMG cap ; 13C label ; 14C label ; mRNA
• 刊名：Bioorganic and Medicinal Chemistry Letters
• 出版年：2012
• 期刊代码：10_0960894x
• 类别：ch
• 出版时间：1 July, 2012
• 卷：22
• 期：13
• 页码：4391-4395
• 文件大小：444 K

摘要

Herein we describe the first simple and short method for specific labeling of mono- and trimethylated dinucleotide mRNA cap analogues with ¹³C and ¹⁴C isotopes. The labels were introduced within the cap structures either at the N7 for monomethylguanosine cap or N7 and N2 position for trimethylguanosine cap. The compounds designed for structural and biochemical studies will be useful tools for better understanding the role of the mRNA cap structures in pre-mRNA splicing, nucleocytoplasmic transport, translation initiation and mRNA degradation.