Expression of kininogen mRNAs and plasma kallikrein mRNA by cultured neurons, astrocytes and meningeal cells in the rat brain
- 作者：Takano ; Masaoki ; Horie ; Masato ; Narahara ; Masanori ; Miyake ; Masaharu ; Okamoto ; Hiroshi
- 关键词：Kininogens ; Plasma kallikrein ; Meningeal cells ; Neurons ; Astrocytes
- 刊名：Immunopharmacology
- 出版年：1999
- 期刊代码：43_01623109
- 类别：imm
- 出版时间：December, 1999
- 卷：45
- 期：1-3
- 页码：121-126
- 文件大小：255 K
摘要
Expression of kininogen mRNAs has been studied in cultures of three different types of cells in rat brain, including neurons and astrocytes from cerebral cortex and meningeal cells from the leptomeninges/choroid plexus. T-kininogen mRNA was expressed by meningeal cells, but not by neurons and astrocytes, and the expression in meningeal cells was enhanced by culture with prostaglandin E2 (PGE2) or dibutyryl cAMP (Bt2cAMP). Low-molecular-weight kininogen mRNA was not detected in these cultures of cells, even after treatment with PGE2. Although expression of high-molecular-weight kininogen mRNA was very low in these cultures of cells, PGE2 or Bt2cAMP markedly stimulated its expression in cultures of meningeal cells and slightly in neurons, but not in astrocytes. We also found that expression of plasma kallikrein mRNA was strong in cultures of meningeal cells and slight in astrocytes, but absent in neurons. These results suggest that cells in the leptomeninges/choroid plexus are major sources of kininogens in rat brain which may function as precursor proteins for kinins and/or potent cysteine proteinase inhibitors during cerebral inflammation.