Exploration of the binding proteins of perfluorooctane sulfonate by a T7 phage display screen

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• 作者：Yuka Miyano^a ; Senko Tsukuda^a ; Ippei Sakimoto^b ; Ryo Takeuchi^a ; Satomi Shimura^a ; Noriyuki Takahashi^a ; Tomoe Kusayanagi^a ; Yoichi Takakusagi^a ; Mami Okado^a ; Yuki Matsumoto^a ; Kaori Takakusagi^a ; Toshifumi Takeuchi^a ; Shinji Kamisuki^a ; Atsuo Nakazaki^c ; Keisuke Ohta^b ; Masahiko Miura^b ; Kouji Kuramochi^d ; ^{kuramoch@kpu.ac.jp} ; Yoshiyuki Mizushina^e ; ^f ; Susumu Kobayashi^c ; Fumio Sugawara^a ; Kengo Sakaguchi^a
• 关键词：Perfluorooctane sulfonate ; Solid-phase synthesis ; Phage display ; CD14 ; RELIC
• 刊名：Bioorganic and Medicinal Chemistry
• 出版年：2012
• 期刊代码：9_09680896
• 类别：ch
• 出版时间：1 July, 2012
• 卷：20
• 期：13
• 页码：3985-3990
• 文件大小：959 K

摘要

Perfluorooctane sulfonate (PFOS) is a pollutant widely found throughout nature and is toxic to animals. We created a PFOS analogue on a polyethylene glycol polyacrylamide copolymer and isolated peptides that preferentially bound the PFOS analogue using a T7 phage display system. Bioinformatic analysis using the FASTAskan program on the RELIC bioinformatics server showed several human proteins that likely bound PFOS. Among them, we confirmed binding between PFOS and a recombinant soluble form of monocyte differentiation antigen CD14 (sCD14) by a surface plasmon biosensor. Furthermore, PFOS inhibited TNF-伪 production induced by the sCD14 in mouse macrophage RAW264.7 cells.