Quantitative analysis of topoisomerase II脙脙脙脙 to rapidly evaluate cell proliferation in brain tumors

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• 作者：Oda ; Masashi ; Arakawa ; Yoshiki ; Kano ; Hideyuki ; Kawabata ; Yasuhiro ; Katsuki ; Takahisa ; Shirahata ; Mitsuaki ; et. al.
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2005
• 期刊代码：159_0006291x
• 类别：bio
• 出版时间：June 17, 2005
• 卷：331
• 期：4
• 页码：971-976
• 文件大小：606 K

摘要

Immunohistochemical cell proliferation analyses have come into wide use for evaluation of tumor malignancy. Topoisomerase IIα (topo IIα), an essential nuclear enzyme, has been known to have cell cycle coupled expression. We here show the usefulness of quantitative analysis of topo IIα mRNA to rapidly evaluate cell proliferation in brain tumors. A protocol to quantify topo IIα mRNA was developed with a real-time RT-PCR. It took only 3 h to quantify from a specimen. A total of 28 brain tumors were analyzed, and the level of topo IIα mRNA was significantly correlated with its immuno-staining index (p < 0.0001, r = 0.9077). Furthermore, it sharply detected that topo IIα mRNA decreased in growth-inhibited glioma cell. These results support that topo IIα mRNA may be a good and rapid indicator to evaluate cell proliferate potential in brain tumors.