Synthesis of photoaffinity probe based on the leaf-opening factor from genus Albizzia

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• 作者：Masahiro Okada ; Akira Matsubara ; Minoru Ueda
• 关键词：Albizzia saman ; Nyctinasty ; Leaf-opening factor ; Photoaffinity probe
• 刊名：Tetrahedron Letters
• 出版年：2008
• 期刊代码：109_00404039
• 类别：ch
• 出版时间：2 June 2008
• 卷：49
• 期：23
• 页码：3794-3796
• 文件大小：112 K

摘要

The circadian rhythmic leaf-movements in legumes, called nyctinasty, are regulated by a pair of leaf-closing and -opening factors. Recent fluorescence studies revealed that cis-p-coumaroylagmatine (1), the leaf-opening factor from genus Albizzia, specifically bound to motor cells in a joint of leaf and stem. In order to identify the receptor protein, which is expected to be involved in the plasma membrane of the cell, we designed and synthesized a photoaffinity probe (2), containing a photoreactive azide group on the aromatic ring and FLAG segment as a peptide antigen for immunoprecipitation.