Insights into RNA Biology from an Atlas of Mammalian mRNA-Binding Proteins

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• 作者：Alfredo Castello¹ ; ⁴ ; Bernd Fischer¹ ; ⁴ ; Katrin Eichelbaum¹ ; Rastislav Horos¹ ; Benedikt  ; M. Beckmann¹ ; Claudia Strein¹ ; Norman  ; E. Davey¹ ; David  ; T. Humphreys² ; Thomas Preiss² ; ³ ; Lars  ; M. Steinmetz¹ ; Jeroen Krijgsveld¹ ; ^{jeroen.krijgsveld@embl.de} ; Matthias  ; W. Hentze¹ ; ² ; ^{hentze@embl.de}
• 刊名：Cell
• 出版年：2012
• 期刊代码：50_00928674
• 类别：med
• 出版时间：8 June, 2012
• 卷：149
• 期：6
• 页码：1393-1406
• 文件大小：1422 K

摘要

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Summary

RNA-binding proteins (RBPs) determine RNA fate from synthesis to decay. Employing two complementary protocols for covalent UV crosslinking of RBPs to RNA, we describe a systematic, unbiased, and comprehensive approach, termed 鈥渋nteractome capture,鈥?to define the mRNA interactome of proliferating human HeLa cells. We identify 860 proteins that qualify as RBPs by biochemical and statistical criteria, adding more than 300 RBPs to those previously known and shedding light on RBPs in disease, RNA-binding enzymes of intermediary metabolism, RNA-binding kinases, and RNA-binding architectures. Unexpectedly, we find that many proteins of the HeLa mRNA interactome are highly intrinsically disordered and enriched in short repetitive amino acid motifs. Interactome capture is broadly applicable to study mRNA interactome composition and dynamics in varied biological settings.