Hyperglycemia and oxidative stress in cultured endothelial cells - a comparison of primary endothelial cells with an immortalized endothelial cell line

详细信息	查看全文 | 推荐本文 |

• 作者：Susanne Karbach^a ; ^b ; ¹ ; Thomas Jansen^a ; ¹ ; Sven Horke^c ; Tjebo Heeren^a ; Alexander Scholz^a ; Meike Coldewey^a ; Angelica Karpi^a ; Michael Hausding^a ; Swenja Kr&ouml ; ller-Sch&ouml ; n^a ; Matthias Oelze^a ; Thomas Mü ; nzel^a ; Andreas Daiber^a ; ^{andreas.daiber@bioredox.com}
• 关键词：Diabetes ; Oxidative stress ; Endothelial Dysfunction ; 2-hydroxyethidium ; cGMP
• 刊名：Journal of Diabetes and its Complications
• 出版年：2012
• 期刊代码：154_10568727
• 类别：med
• 出版时间：May-June, 2012
• 卷：26
• 期：3
• 页码：155-162
• 文件大小：1117 K

摘要

Diabetes mellitus is a major risk factor for the development of cardiovascular disease and oxidative stress plays an important role in this process. Therefore, we investigated the effects of hyperglycemia on the formation of reactive oxygen species (ROS) and nitric oxide/cGMP signaling in two different endothelial cell cultures. Human umbilical vein endothelial cells (HUVEC) and EA.hy 926 cells showed increased oxidative stress and impaired NO-cGMP signaling in response to hyperglycemia. The major difference between the two different cell types was the dramatic decrease in viability in HUVEC whereas EA.hy cells showed rather increased growth under hyperglycemic conditions. Starvation led to an additional substantial decrease in viability and increased superoxide formation in HUVEC. Both endothelial cell types, HUVEC and EA.hy 926, may be used as models for vascular hyperglycemia. However, high growth medium should be used to avoid starvation-induced oxidative stress and cell death.