Simultaneous determination of tyrosine, tryptophan and 5-hydroxytryptamine in serum of MDD patients by high performance liquid chromatography with fluorescence detection

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• 作者：Mu Sa^a ; Li Ying^a ; Ai-Guo Tang^a ; ^{aiguotang@yahoo.com.cn} ; Le-Dong Xiao^b ; Ya-Ping Ren^a
• 关键词：HPLC-FLD ; high performance liquid chromatography with fluorescence detection ; Tyr ; tyrosine ; Trp ; tryptophan ; 5-HT ; 5-hydroxytryptamine ; MDD ; major depressive disorder
• 刊名：Clinica Chimica Acta
• 出版年：2012
• 期刊代码：54_00098981
• 类别：med
• 出版时间：14 June, 2012
• 卷：413
• 期：11-12
• 页码：973-977
• 文件大小：214 K

摘要

Background

Tyrosine (Tyr), Tryptophan (Trp) and 5-hydroxytryptamine (5-HT) are important amino acids in vivo and have been hypothesized to be involved in many mental disorders. We developed a rapid and sensitive HPLC method for simultaneous measurement of serum Tyr, Trp and 5-HT and explored the clinical significances of Tyr, Trp and 5-HT and the 5-HT/Trp ratio for patients with major depressive disorder (MDD) disease.

Methods

Serum samples were deproteinized by 5%perchloric acid and separated on an Atlantis C18 column (4.6 脳 150 mm, 5 渭m) with the mobile phase consisting of 0.1 mol/l KH₂PO₄ and methanol (85:15, V/V).The eluates were monitored by the fluorescence detection with programmed wavelength.

Results

Analysis was achieved in < 12.0 min. The limits of quantification were 0.014, 0.005, and 0.024 渭mol/l for Tyr, Trp and 5-HT, respectively. Reproducibility and recovery were satisfactory. Tyr, Trp and 5-HT and the 5-HT/Trp ratio were significantly decreased in patients with MDD.

Conclusions

In diseases, like MDD, Tyr, Trp and 5-HT play an important role. This method can potentially be applied as prognostic or diagnostic tool or even to follow the evolution of the illness or of the treatment.